Our vision is to develop our sensitive and clinical friendly targeted nanosized-based system to detect earlier of the most aggressive kinds of breast cancer and other HER2 positive tumors such as
ovary and stomach. In continuation our vision is to simultaneously use this technology for detection and therapy by loading chemotherapeutics. Furthermore, we would like to expand this radionuclide imaging system to other life-threatening cancers.
Our aim is to develop a clinical friendly detection-based method to detect HER2 positive breast tumors both at the early stages of occurrence and well as detection of metastatic disease .
we are using exosomes, which are produced by HEK 293 cells. Exosomes are targeted against HER2 receptors by genetic manipulation. Targeted exosomes are labeled by gamma emitting radionuclides to detect tumors even in deep organs by PET/SPECT imaging.
Our nanosized based detection system called exosomes are targeted against HER2 positive tumors. These exosomes are genetically modified to express a high affinity ligand against the HER2 receptor on their surface. The radionuclides [99mTc]Tc-HMPAO and [111In]In-oxine are loaded inside these tiny vesicles to detect tumors by the SPECT system. SPECT is a non- invasive approach for detecting tumor tissues with high sensitivity, efficient spatial resolution and quantification.
Aim: Here, we established a reliable strategy for generation and characterization of targeted radiolabeled exosomes for the detection of HER2-positive cells quantitatively. Materials & methods: Targeted exosomes (T-exos) were radiolabeled by two different radiotracers, [99mTc]Tc-HMPAO or [111In]In-oxine. The labeling efficiency and stability were assessed using exosome exclusive spin columns. HER2-positive and -negative cells were treated with [111In]In-oxine-exosomes after 3 and 24 h. Results: [111In]In-oxine labeling did not change the binding ability and general features of the exosomes. With [111In]In-oxine, 70% labeling efficiency and 78% radiochemical stability over 24 h were achieved. [111In]In-oxine-T-exos showed greater uptake by HER2-positive cells compared with untargeted exosomes. Conclusion: [111In]In-oxine-T-exos could potentially be used as an effective imaging tool for HER2 expression